We herein present an integrated multifunctional microelectrode array (mMEA) chip harnessed with multiple measurement modalities of both neural-electrical and neural-chemical recordings to enable simultaneous monitoring of action potential and the level of the specific neurotransmitter. A dopamine sensor modality fabricated in interdigitated electrodes (IDE) fashion was realized and characterized, subsequently applied to trace dopamine exocytosis in PC12 cells cultured on such mMEA chip.
Parameter Value
N umber of IDE pairs 8
Number of MEs 40
Number of GEs 4
Number of pads 60
Number of digit/ IDE collector electrode 6
Number of digit/ IDE generator electrode 6
Length of Chip (A) (cm) 5.7
Width of Chip (B) (cm) 5.7
IDE collector/generator length (C) (μm) 282
IDE collector/generator width (D) (μm) 50
ME radius (E) (μm) 30
GE radius (F) (μm) 60
Distance of ME interspace (G) (μm) 200
Length of electrode digit (H) (μm) 50
Width of electrode digit(I) (μm) 12
Distance of electrodes digit interspace (J) (μm) 48
Width of wire (K) (μm) 30